Dig nucleic acid detection kit manual

genesig® Easy DNA/RNA Extraction Kit Handbook HB Published date: 12/07/ 3. Product information The basic principle The genesig Easy Extraction kit is designed for the isolation of DNA/RNA from a huge range of sample types. This kit provides reagents and magnetic beads for isolation of 50 samples of approximately –μl. Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. The Thermo Scientific Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications. Nearly any DNA or RNA method based on nitrocellulose or nylon membranes and biotinylated probes can be detected with sensitivity equivalent to traditional.

DIG dUTP is incorporated every nucleotides into the newly synthesized DNA. This density of haptens in the DNA yields the highest sensitivity in the detection reaction. Test principle The DIG DNA Labeling and Detection Kit uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme. DIG Luminescent Detec-tion Kit for Nucleic acids 1 kit (50 blots) 11 DIG Nucleic Acid Detection Kit 40 blots (10× 10 cm) 11 PCR DIG Probe Synthesis Kit 25 reactions 11 PCR DIG Labeling Mix (for analysis of PCR products) 2× l 11 DIG Gel Shift Kit 2nd Generation 1 kit 03 DigoxigenindUTP. DIG Luminescent Detection Kit Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. Cat. No. 1 Kit for the detection of 50 blots of cm² Store at 15 to 25° C Instruction Manual Version 2, March www.doorway.ru Page 1 Thursday, Ma AM.

DIG-labeled DNA, RNA or oligonucleotide probes are detected, after hybridization to target nucleic acids, by enzyme-linked immunoassay using an antibody-. PCR amplification can turn a few molecules of a specific target nucleic acid (too little to be analyzed directly or used in biochemical reactions) into as. Store this kit at J15 to J25°C. Kit for 12 labeling reactions of 10 ng-3 µg. DNA and detection of 24 blots of 10 × 10 cm2. Instruction Manual.